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Generation of a red pigment in the yeast vacuole can be observed visually in yeast colonies in this assay if the N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase (Ade1) or the phosphoribosylaminoimidazole carboxylase (Ade2) is not functional
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Placing the composition in front of a dark background highlights the anatomical details of the skeleton head, as well as Van Goghs handling of color while working with a darker, limited palette
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